A label-free electrochemical strategy for highly sensitive methyltransferase activity assays.
نویسندگان
چکیده
A new electrochemical strategy for the simple and ultrasensitive evaluation of DNA methyltransferase (MTase) activity was developed based on electrocatalytic oxidation of ascorbic acid by graphene. In addition, the suitability of this sensing platform for MTase inhibitor screening was also demonstrated.
منابع مشابه
Label-free electrochemical biosensors for food and drug application
In food sector, there is a huge demand for rapid, reliable, user & eco-friendly biosensors to analyse the quality and safety of food products. Biosensor based methodology depends upon the recognition of a specific antigens or receptors by corresponding antibodies, aptamers or high-affinity ligands. The first scientifically commercialised sensors were the electrochemical sensors used for the ana...
متن کاملLabel-free electrochemical biosensors for food and drug application
In food sector, there is a huge demand for rapid, reliable, user & eco-friendly biosensors to analyse the quality and safety of food products. Biosensor based methodology depends upon the recognition of a specific antigens or receptors by corresponding antibodies, aptamers or high-affinity ligands. The first scientifically commercialised sensors were the electrochemical sensors used for the ana...
متن کاملLabel-free molecular beacon-based quadratic isothermal exponential amplification: a simple and sensitive one-pot method to detect DNA methyltransferase activity.
We developed a one-pot label-free molecular beacon-mediated quadratic isothermal exponential amplification strategy (LFMB-QIEA) for simple, rapid and sensitive DNA methyltransferase (MTase) activity detection.
متن کاملElectrochemical assay for the signal-on detection of human DNA methyltransferase activity.
Strategies to detect human DNA methyltransferases are needed, given that aberrant methylation by these enzymes is associated with cancer initiation and progression. Here we describe a nonradioactive, antibody-free, electrochemical assay in which methyltransferase activity on DNA-modified electrodes confers protection from restriction for signal-on detection. We implement this assay with a multi...
متن کاملMethylation-blocked enzymatic recycling amplification for highly sensitive fluorescence sensing of DNA methyltransferase activity.
Herein, using DNA adenine methylation (Dam) methyltransferase (MTase) as a model analyte, a novel fluorescence sensing strategy was developed for facile, rapid and highly sensitive detection of the activity and inhibition of the target based on methylation-blocked enzymatic recycling amplification. In this sensing system, nicking endonuclease Nt.AlwI with the methylation-sensitive property was ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Chemical communications
دوره 51 24 شماره
صفحات -
تاریخ انتشار 2015